Journal: International Journal of Molecular Sciences

Article Title: Pharmaceutical Functionalization of Monomeric Ionic Liquid for the Preparation of Ionic Graft Polymer Conjugates

doi: 10.3390/ijms232314731

Figure Lengend Snippet: Viability of NHDF and BEAS-2B cell lines treated by graft copolymers at different concentrations after 72 h of incubation.

Article Snippet: Human bronchial epithelial cells (BEAS-2B) and normal human dermal fibroblasts (NHDF) were purchased from ATCC (Cat# ATCC ® CRL-9609; Manassas, VA, USA).

Techniques: Incubation

Journal: International Journal of Molecular Sciences

Article Title: Pharmaceutical Functionalization of Monomeric Ionic Liquid for the Preparation of Ionic Graft Polymer Conjugates

doi: 10.3390/ijms232314731

Figure Lengend Snippet: Microscopic images by Live Cell Analyzer for control vs. treated ( a ) NHDF ( b ) BEAS-2B cells by G_CLX¯ G_FUS¯ and G_CLX¯/FUS¯ at 100 and 3 µg/mL.

Article Snippet: Human bronchial epithelial cells (BEAS-2B) and normal human dermal fibroblasts (NHDF) were purchased from ATCC (Cat# ATCC ® CRL-9609; Manassas, VA, USA).

Techniques: Control

Journal: Infection and Drug Resistance

Article Title: Synergism of cationic antimicrobial peptide WLBU2 with antibacterial agents against biofilms of multi-drug resistant Acinetobacter baumannii and Klebsiella pneumoniae

doi: 10.2147/IDR.S215084

Figure Lengend Snippet: Viability of Vero cells and human skin fibroblasts exposed to WLBU2. Vero cells or human skin fibroblasts were incubated with different concentrations of WLBU2 or media alone (control) for 24 hrs. Cell viability was determined using the MTT assay. Experiments were run in triplicates. Bars represent means±standard error of the mean. WLBU2 concentrations in µM correspond to 5.313, 10.625, 21.250, 42.500, 85, 170, 340, 680 µg/mL, respectively.

Article Snippet: Eukaryotic cell viability experiments utilized Vero cells (African green monkey kidney epithelial cells; ATCC CCL-81) and human skin fibroblast cells (ATCC PCS-201–012).

Techniques: Incubation, Control, MTT Assay

Journal: Infection and Drug Resistance

Article Title: Synergism of cationic antimicrobial peptide WLBU2 with antibacterial agents against biofilms of multi-drug resistant Acinetobacter baumannii and Klebsiella pneumoniae

doi: 10.2147/IDR.S215084

Figure Lengend Snippet: Viability of Vero cells and skin fibroblasts exposed to WLBU2 combined with antimicrobial agents. Vero cells or human skin fibroblasts were incubated with WLBU2 at 12.500 µM (42.5 µg/mL) or 25 µM (85 µg/mL) combined with antimicrobial agents at the indicated concentrations for 24 hrs. Cell viability was determined using the MTT assay. Experiments were run in triplicates. Bars represent means±standard error of the mean.

Article Snippet: Eukaryotic cell viability experiments utilized Vero cells (African green monkey kidney epithelial cells; ATCC CCL-81) and human skin fibroblast cells (ATCC PCS-201–012).

Techniques: Incubation, MTT Assay

Journal: PLOS ONE

Article Title: Borrelia burgdorferi -mediated induction of miR146a-5p fine tunes the inflammatory response in human dermal fibroblasts

doi: 10.1371/journal.pone.0286959

Figure Lengend Snippet: The lists of predicted miRNAs and the overrepresented DE gene targets were filtered; >1 interaction, P<0.05, FDR<10%. (A) Upstream miRNA regulation of differentially expressed (DE) genes in erythema migrans skin lesions of early Lyme disease patients. DE genes (circles) reported by Marques et al . and predicted upstream miRNA regulators (squares) that had an overrepresented number of DE gene targets. Red and blue circles represent the reported upregulated and downregulated genes, respectively. Light red and light blue squares represent predicted upregulated and downregulated miRNAs, respectively. Grey squares represent miRNAs that have an overrepresented number of gene targets in both the upregulated and downregulated sets of DE genes. (B) Upstream miRNA regulation of DE genes from primary human dermal fibroblasts (HDFs) in response to stimulation with B . burgdorferi sensu lato for 24 hours. Network representing strong validated interactions for miRNAs that had an overrepresented number of targets within the upregulated genes (red circles) reported by Meddeb et al . . Light blue squares represent presumably down-regulated miRNAs. (C) Venn Diagram outlines common and unique predicted upstream miRNA regulators for the two sets of DE genes reported for skin lesions of early Lyme disease patients (skin) and B . burgdorferi stimulated HDFs (fibroblasts).

Article Snippet: Primary Dermal Fibroblast; Normal, Human, Adult (HDFa, PCS-201-012, ATCC) were grown in fibroblast basal medium (PCS-201-030, ATCC), supplemented with fibroblast growth kit-serum-free (PCS-201-040, ATCC) and 10% fetal bovine serum (FBS) to passage 3 and aliquots stored at -80°C.

Techniques:

Journal: PLOS ONE

Article Title: Borrelia burgdorferi -mediated induction of miR146a-5p fine tunes the inflammatory response in human dermal fibroblasts

doi: 10.1371/journal.pone.0286959

Figure Lengend Snippet: Stimulation of human dermal fibroblasts with B . burgdorferi results in increased expression of genes important for inflammation as well as the anti-inflammatory microRNA, miR146a-5p. Regulation by miR146a-5p reduces inflammatory gene expression levels perhaps by targeting mechanisms of NF-ϰB activation and/or the inflammatory gene transcripts directly.

Article Snippet: Primary Dermal Fibroblast; Normal, Human, Adult (HDFa, PCS-201-012, ATCC) were grown in fibroblast basal medium (PCS-201-030, ATCC), supplemented with fibroblast growth kit-serum-free (PCS-201-040, ATCC) and 10% fetal bovine serum (FBS) to passage 3 and aliquots stored at -80°C.

Techniques: Expressing, Gene Expression, Activation Assay